Isolation by Differential and Zonal Centrifugation of Minicells Segregated by Escherichia coli
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چکیده
منابع مشابه
Isolation by differential and zonal centrifugation of minicells segregated by Escherichia coli.
Minicells segregated from Escherichia coli chi925 carrying a drug-resistance plasmid were separated from nucleated cells by differential centrifugation and purified by rate-zonal centrifugation in sucrose gradients. Minicells purified in this way were capable of donating the plasmid to nucleated cells. They also incorporated thymidine, uridine and methionine into macromolecules. Methods are des...
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The isolated, formaldehyde-fixed nucleoid of E. coli has been analyzed by isopycnic centrifugation in CsCl density gradients. The membrane-free nucleoid bands at a density of 1.69 +/- 0.02 g/cm3. The membrane-associated nucleoid bands at a density of 1.46 +/- 0.02 g/cm3. Both species sediment to equilibrium as nearly monodisperse bands in CsCl, suggesting that the nucleoid components of DNA, RN...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1979
ISSN: 0022-1287
DOI: 10.1099/00221287-111-2-387